Journal: iScience

Article Title: Human skin CD141 + dendritic cells regulate cutaneous immunity via the neuropeptide urocortin 2

doi: 10.1016/j.isci.2023.108029

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Article Snippet: Monoclonal anti-Human CD278 (ICOS)-VioGreen (Clone: REA192) , Miltenyi Biotec , Cat # 130-100-740.

Techniques: Purification, Recombinant, Staining, Concentration Assay, Sterility, Reverse Transcription, Multiplex Assay, Labeling, Expressing, Transfection, Software

Journal: Journal of Immunology Research

Article Title: Implementation of Mass Cytometry for Immunoprofiling of Patients with Solid Tumors

doi: 10.1155/2019/6705949

Figure Lengend Snippet: Panel of antibodies for mass cytometry.

Article Snippet: 25 , CD278 (ICOS) , 143Nd , DX29 , Surface , Fluidigm.

Techniques: Cytometry, Staining

Journal: iScience

Article Title: Human skin CD141 + dendritic cells regulate cutaneous immunity via the neuropeptide urocortin 2

doi: 10.1016/j.isci.2023.108029

Figure Lengend Snippet:

Article Snippet: The following antibodies were used in different combinations: CD3-FITC (eBioscience); CD19-FITC, CD20-FITC, CD56-FITC, CD25-PE, CCR4-PECy7, CCR6-PerCp/Cy5.5,HLA-ABC-PE, CD3 - BUV395 (BD); CLEC9A-PE, CD1c-PE, CD1c-APC, CD141-PE,CD141-APC, CD278 (ICOS)-VioGreen, CD127 – APC, CD25 -PE and CD45 -VioBright515 (Miltenyi); CD3-FITC, PD-L1-PE and CD45-PECy7 (eBioscience); CD11c-PerCp/Cy5.5, CD4 -BV785, CD8a - BV650, CTLA-4 - BV605, FoxP3 - BV421 and Ki-67 - BV711 (BioLegend); CCR10-PE (R&D Systems); CD14-FITC, CD83-FITC, CD86-FITC, CD40-FITC and HLA-DR-PE (Invitrogen).

Techniques: Purification, Recombinant, Staining, Concentration Assay, Clinical Proteomics, Sterility, cDNA Synthesis, Reverse Transcription, Multiplex Assay, Labeling, Expressing, Transfection, Software

Journal: STAR Protocols

Article Title: Protocol to characterize immune cell subpopulations in cerebrospinal fluid of patients with neuroinflammatory diseases using mass cytometry

doi: 10.1016/j.xpro.2024.103038

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Article Snippet: ICOS/CD278 (C398.4A)-151Eu (1:50) , Standard BioTools , Cat#3151020B.

Techniques: Recombinant, Labeling, Blocking Assay, Software, Cytometry, Flow Cytometry, Single-cell Analysis, Gene Expression

Journal: Inflammation

Article Title: Methylprednisolone Modulates the Tfr/Tfh ratio in EAE-Induced Neuroinflammation through the PI3K/AKT/FoxO1 and PI3K/AKT/mTOR Signalling Pathways

doi: 10.1007/s10753-024-02099-y

Figure Lengend Snippet: Flow cytometry analysis of different subsets of splenic Tfr and Tfh cells in the control, Pre-EAE, P-EAE, and EAE + MP groups. A The percentages of CD4 + CXCR5 + Foxp3 + Tfr and CD4 + CXCR5 + Foxp3-Tfh cells in the spleens of EAE mice in these four groups. B The percentages of CD4 + CXCR5 + Foxp3-ICOS + and CD4 + CXCR5 + Foxp3-PD-1 + Tfh cells in the spleens of EAE mice in these four groups. n = 5 per group. The data are presented as the mean ± SEM. * P < 0.05, ** P < 0.01*** P < 0.001.

Article Snippet: The membranes were blocked in a solution containing TBST with 5% skim milk for one hour and then incubated respectively with anti-PI3K (Cell Signaling Technology, 34,050), anti-phospho-AKT (Affinity, AF0016), anti-AKT (Affinity, AF6261), anti-phospho-FoxO1 (Cell Signaling Technology, 9464), anti-FoxO1(Affinity, AF3417), anti-phospho-mTOR (Cell Signaling Technology, 5536), anti-mTOR (Cell Signaling Technology, 2983), CXCR5 (ABclonal, A8950), Foxp3 (Affinity, AF6544), ICOS (ABclonal, A1811), PD-1 (Cell Signaling Technology, 84,651), TGF-β1 (Proteintech, 21,898–1-AP), IL-21 (Affinity, DF4818), GFAP (Cell Signaling Technology, 3670), Iba-1 (Cell Signaling Technology, 17,198), Arg-1 (Proteintech,16,001–1-AP), iNOS (Proteintech, 18985–1-AP) and GAPDH (BOSTER, BM3874) antibodies overnight at 4 °C.

Techniques: Flow Cytometry, Control

Journal: Inflammation

Article Title: Methylprednisolone Modulates the Tfr/Tfh ratio in EAE-Induced Neuroinflammation through the PI3K/AKT/FoxO1 and PI3K/AKT/mTOR Signalling Pathways

doi: 10.1007/s10753-024-02099-y

Figure Lengend Snippet: EAE mice were classified into the control group, the Pre-EAE group, the P-EAE group and the EAE + MP group. Representative blots bands of CXCR5, PD-1, iCOS, TGF-β1, FoxP3, and IL-21 in the spinal cords of EAE mice were analysed. n = 3 per group. The data are presented as the mean ± SEM. * P < 0.05, ** P < 0.01.*** P < 0.001, and **** P < 0.0001.

Article Snippet: The membranes were blocked in a solution containing TBST with 5% skim milk for one hour and then incubated respectively with anti-PI3K (Cell Signaling Technology, 34,050), anti-phospho-AKT (Affinity, AF0016), anti-AKT (Affinity, AF6261), anti-phospho-FoxO1 (Cell Signaling Technology, 9464), anti-FoxO1(Affinity, AF3417), anti-phospho-mTOR (Cell Signaling Technology, 5536), anti-mTOR (Cell Signaling Technology, 2983), CXCR5 (ABclonal, A8950), Foxp3 (Affinity, AF6544), ICOS (ABclonal, A1811), PD-1 (Cell Signaling Technology, 84,651), TGF-β1 (Proteintech, 21,898–1-AP), IL-21 (Affinity, DF4818), GFAP (Cell Signaling Technology, 3670), Iba-1 (Cell Signaling Technology, 17,198), Arg-1 (Proteintech,16,001–1-AP), iNOS (Proteintech, 18985–1-AP) and GAPDH (BOSTER, BM3874) antibodies overnight at 4 °C.

Techniques: Control